TK 89 soybean

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Derivation of TK- clones from revertant TK+ mammalian cells.

In order to obtain a large collection of Chinese hamster cell clones defective in thymidine kinase (TK(-)), BrdU(r) selection experiments have been performed on wild-type and revertant TK(+) cell lines. No clones (< 10(-9)) were obtained from the wild-type TK(+) cell line by single-step selection. In contrast, revertant TK(+) clones readily gave rise to stable TK(-) derivatives (1 - 2 x 10(-4))...

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ژورنال

عنوان ژورنال: Canadian Journal of Plant Science

سال: 1991

ISSN: 0008-4220,1918-1833

DOI: 10.4141/cjps91-122